FertiCult Aspiration medium

Cell culture medium for oocyte pick-up

FertiCult™ Aspiration medium is a ready to use formulation for oocyte pick-up and follicular flushing.

Information about the composition of the product can be found in the material safety data sheet.

Regulatory information

Europe: CE-marked – Brazil: registered

Product order codes

ASPI020 : 5x 20mL FertiCult Aspiration Medium
ASPI100 : 5x 100mL FertiCult Aspiration Medium

pH	7.3-7.6
Osmolality	270-290mOsm/kg
Sterility	Sterile
Endotoxin	< 0.25EU/mL
Mouse embryo test	≥ 80% blastocysts after 96h incubation
Shelf life	18 months from date of produce

Component

Benefit

HEPES (18 mM)

HEPES stabilizes the pH under air (Clark and Swain 2014), therefore CO₂ incubation is not required.

Heparin sodium (2.5 IU/ml)

Heparin is added to the media to avoid blood clot formation in the tubing during aspiration and/or flushing of oocytes in Assisted Reproductive Technologies.

Heparin is naturally present in the uterine, tubal and follicular fluid (Agostini 2006), (Yanagishita 1994).

Prospective randomized trial revealed that heparinized saline or culture medium (2 IU/ml) allow for successful follicle flushing (Biljan, et al. 1997).

Falah, KM.; Banna, H.; Aghaways, HA.; Zangana, RA.; , Using in-vitro maturation of immature oocytes retrieved from poor responder patients to improve pregnancy outcomes in Sulaimani government region, Iraq., European Scientific Journal (2014),Vol.10,No.33,pp.195-211

Agostini, Arian I. de., An unexpected role for anticoagulant heparin sulfate proteoglycans in reproduction., Swiss Med Wkly (2006),Vol.136,No.37-38,pp.349-352

Biljan, M.M., Dean,N., Hemmings,R., Bissonnette,F., and Tan,S.L. , Prospective randomized trial of the effect of two flushing media on oocyte collection and fertilization rates after in vitro fertilization., Fertil. Steril. (1997),Vol.68,pp.1132-1134

Clark, N.A. and Swain, J.E., Buffering systems in IVF., Culture media, Solutions, and Systems in Human ART, by P. Quinn (2014),pp.30-46
Yanagishita, M., Proteoglycans and hyaluronan in female reproductive organs., EXS (1994),Vol.70,pp.179-90

Frequently Asked Questions

Certificate Of Analysis

The success of human infertility treatments depends heavily on high-quality cell culture media in each step of the procedure.